Fatty-acylated sericin {1:0.7 molar ratio of sericin (Mr 18,700) to oleic acid} was prepared by lipase-catalyzed solid-phase synthesis in n-hexane containing oleic acid to endow sericin with interfacial properties. Acylation with oleic acid was confirmed by 1H-NMR. The fatty-acylated sericin exhibited superior emulsifying activity index and emulsion stability in the presence of 0?0.5 M NaCl, in a temperature range of 30?80 °C and pH range of 2?7, as compared with the control sericin. The fatty-acylated sericin (1:0.4 molar ratio) prepared by using low-molecular-weight sericin (Mr 5,000) also exhibited superior emulsifying properties. The affinity of the fatty-acylated sericin to a hydrophobic surface as evaluated by a biomolecular interaction analyzer was about twice as much as that of the control sericin. The fatty-acylated sericin showed retarded water vaporization, similar to the control sericin, indicating good retention of moistness, and was adsorbed four times as much to defatted wool with little desorption as compared with the control sericin.
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机译：通过在含油酸的正己烷中通过脂肪酶催化固相合成，制备脂肪酰化的丝胶蛋白（丝胶蛋白（Mr 18,700）与丝胶蛋白的摩尔比为1：0.7），赋予丝胶蛋白以界面特性。通过1 H-NMR确认了油酸的酰化。与对照丝胶相比，在0〜0.5 M NaCl存在下，在30〜80°C的温度范围和2〜7的pH范围内，脂酰化的丝胶具有良好的乳化活性指数和乳化稳定性。通过使用低分子量水溶蛋白（Mr 5,000）制备的脂肪酰化水溶蛋白（1：0.4摩尔比）也表现出优异的乳化性能。通过生物分子相互作用分析仪评估，脂肪酰化的丝胶蛋白对疏水表面的亲和力约为对照丝胶蛋白的亲和力的两倍。与对照丝胶类似，脂肪酰化的丝胶具有延迟的水汽化作用，表明具有良好的保湿性，并且与对照丝胶相比，脱脂羊毛的吸附量是脱脂羊毛的四倍。
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